In Vitro Trial
In the present study, the human pathogens such as Escherichia coli, Staphylococcus aureus, Klebsiellapneumoniae and Bacillus cereus were isolated and the effects of various fractions of Ceratopteristhalictroides, Christelladentata, Lygodiumflexuosum, Pterisvittata and Salviniamolestaon these organisms were tested. By column chromatography the extracts of various solvents are made and they are petroleum ether, chloroform, acetone and methanol extracts.Using these extracts antibacterial activity of CeratopteristhalictroidesChristelladentata, Lygodiumflexuosum. Pterisvittata and Salviniamolestawere tested. From the present investigation. It become clear that the five selected pteridophytes such as Ceratopteristhalictroides Christelladentata, Lygodiumflexuosum, Pterisvittata and Salviniamolesta possess antibacterial activity. The activity may be due to the presence of some phytochemicals, which can be later identified. The most effective result of these study point that the Petroleum ether extract of Salviniamolesta against E. coli shows 88 % of inhibition against E. coli. The Petroleum ether extract of Salviniamolesta shows the 104% of inhibitory effect against Klebsiellapneumoniae.The Petroleum ether extract of all five plants show 82%inhibition against Staphylococcus aureus and Petroleum ether extract of Lygodiumflexousum show 77%of inhibitory effect against Bacillus cereus.These results show the petroleum ether extract of plants are most useful and most effective drug against the selected microbes. Especially the Petroleum ether extract of Salviniamolesta shows the 104 % of inhibitory effect against Klebsiellapneumoniae. So the inhibitory compounds are dissolved in the Petroleum ether extracts. The Petroleum ether extracts of plants will be effective against gram negative bacteria. The pure compound extraction and application in animals is necessary for the future study.
P. V. Anto, K V Greeshma, N. A. Santhosh
International journal of innovative research and development