Finding
Conclusion The joint use of alcohol,high fat diet and carbonyl iron can induce a reliable rat animal model with alcoholic hepatitis.
Paper
Therapeutic evaluation of recombinant human interleukin-22 on alcoholic hepatitis in rats
Abstract
Objective To construct a reliable rat animal model with alcoholic hepatitis and investigate the effect of IL-22 on such model.Methods Sixty male Wistar rats were randomly divided into two groups:normal control group and alcohol group.The combination of alcohol,high fat diet as well as carbonyl iron was used to induce alcoholic hepatitis in rats.The level of serum aminotransferases and hepatic histopathology were examined at 16,24 and 32 weeks respectively.When histopathological changes of alcoholic hepatitis occurred,IL-22 was given by the tail vein at the dose of 0.5 mg/kg for a total of 10 times.At the end of the experiment,the changes of serum ALT,AST,GGT,TG and CHO as well as hepatic pathology in different groups were detected respectively.Results At 32 weeks,histopathological changes of alcoholic hepatitis occurred and the serum ALT,AST,GGT,TG and CHO were significantly elevated.Treatment with IL-22 could reverse the above adverse changes.Conclusion The joint use of alcohol,high fat diet and carbonyl iron can induce a reliable rat animal model with alcoholic hepatitis.IL-22 can significantly decrease the elevation of serum enzymes and improve histopathology of alcoholic hepatitis in the rat model,suggesting that IL-22 is a potential drug for alcoholic hepatitis.
Authors
Xu Dong-gang
Journal
Military Medical Sciences