imaging analysis, the well-characterized proinflammatory marker MRP8/14 was detected by FMT using an anti-MRP14 antibody coupled to Cy5.5-NHS-ester. Fluorochromes (antiMRP14-Cy5.5) were injected at amounts of 2 nm Cy5.5 per animal at day 0, 4 and 8. In addition, we performed In Vivo ultrasound microscopy to assess morphology and function of the distal parts of the colon. Peristalsis and colon-wall thickness were assessed. Results: DSS-treated animals showed a significantly increased overall weight loss and revealed increased signs of colonic inflammation in comparison with the control group (p<0.01). Mucosal MPO-activity and IL-1beta mRNA-expression were significantly increased in DSStreated animals (p<0.05). Confirmatively, the intensity of colonic inflammation as measured by 3 D reconstruction of anti-MRP14 FMT was significantly higher in DSS-treated animals (238.4 pmol) than in control mice (147.8 pmol, p<0.05). Peristalsis was substantially diminished in the transverse, descendens and rectum. The rectal lumen was significantly enlarged (p<0.05). The wall of the proximal rectum was significantly thicker as compared to the control animals (control: 0.35 mm, DSS-treated mice: 1.2 mm, p<0.05). Conclusions: We demonstrate for the first time that the course of DSS colitis measured by the common inflammatory markers correlates adequately by the findings of fluorescence-mediatedmolecular tomography (FMT) and high-resolution ultrasound imaging. Thus, these new techniques are appropriate tools for In Vivo imaging of murine colitis offering the opportunity of several In Vivo follow-up investigations.
Chong Shen, G. Hertogh, E. Dilissen