Finding
Such distances are in agreement with what is required for maturation of mammalian pre-mRNAs.
Paper
Cloning of the mouse 25-hydroxyvitamin D3-1α-hydroxylase (CYP1α) gene
Abstract
Abstract A genomic clone for 25-hydroxyvitamin D3-1α-hydroxylase (1α-hydroxylase) was isolated from a mouse embryonic stem cell P1 genomic library. It contains nine exons spanning 4.8 kb from the transcriptional start site. All the intron insertion sites are identical to that of the human 1α-hydroxylase and human vitamin D3 25-hydroxylase genes. A polyadenylation signal AUUAAA that differs from the consensus sequence at the second residue was identified 16 bp downstream of the 3′ end of the previously reported mouse cDNA. This element is the only common natural variant described. The 3′ end of the gene was determined using a RACE technique. Three poly(A) addition sites were observed 12, 15 and 22 bases from the AUUAAA element. Such distances are in agreement with what is required for maturation of mammalian pre-mRNAs. This molecular cloning makes possible the generation of transgenic mice in order to further investigate the role and importance of the 25-hydroxyvitamin D3-1α-hydroxylase (CYP1α).
Authors
C. Kimmel-Jehan
Journal
Biochimica et Biophysica Acta