Finding
The epitope-imprinted MNPs-based SELEX is straightforward and generally applicable for a wide range of target proteins, providing a promising aptamer selection tool for aptamer-based research and real-world applications.
Paper
Epitope-Imprinted Magnetic Nanoparticles as a General Platform for Efficient in Vitro Evolution of Protein-Binding Aptamers.
In Vitro Trial
Citations: 9
Full textAbstract
Aptamers are usually created by in vitro selection using a strategy called systematic evolution of ligands by exponential enrichment (SELEX). Although numerous SELEX alternatives with improved selection efficiency have been developed, the overall success rate of SELEX at present is still not very ideal, which remains a great obstacle to aptamer-based research and application. In this study, a facile and efficient SELEX approach was developed for in vitro selection of protein-binding aptamers, using epitope-imprinted magnetic nanoparticles (MNPs) that exhibit highly favorable binding properties as a general affinity platform. As a proof of the principle, myoglobin (Mb) and β2-microglobulin (B2M) were employed as two target proteins. Two satisfied aptamers towards each target protein, with dissociation constant at the 10-8 M level and cross-reactivity less than 16.5%, were selected within 3 rounds, taking only 1 day. A dual aptamer-based fluorescence sandwich assay was constructed using a pair of selected aptamers. The resulting assay allowed for the quantitative detection of Mb in human serum and the differentiation of acute myocardial infarction patients from healthy individuals. The epitope-imprinted MNPs-based SELEX is straightforward and generally applicable for a wide range of target proteins, providing a promising aptamer selection tool for aptamer-based research and real-world applications.
Authors
Yanyan Ma, Wei Li, Rongrong Xing
Journal
ACS sensors