1. The ehromosomes in the epithelial cells of larval salamanders were prepared for study by the use of the smear method. Both acetocarmine and permanent smears were used.2. The chromosomes of Ainblystoma punctatum consist of a matrix in which lies a coiled or zigzag thread. The thread is double at all stages except metaphase when it is quadruple.3. In occasional cells the chromatids are widely separated during prophase and metaphase. A possible explanation for this condition is suggested.