Synaptosomes were prepared from brains of rats of different age groups and from embryonic chick brains and glycoprotein glycosyl transferase activities of these preparations were compared. It was observed that the glycosyl transferase activities were always associated with the presence of membrane-bound polysomes in these fractions. Furthermore since synaptosomal fractions in which the polysomal contamination was minimal had little or no glycosyl transferase activity, it is concluded that the transferase activity which has been ascribed by other workers to the synaptosomal fraction, can in fact be traced to the presence of membrane-bound polysomes.
E Raghupathy, G K Ko, N A Peterson
Biochimica et biophysica acta