Finding
As the T γ + lymphocytes were considerably enriched in cells having the characteristics of NK cells according to their phenotyping, morphology and NK cell activity, we may assume that NK cells have a low B:A ratio.
Paper
LDH isoenzyme distribution in human Tγ lymphocytes
Abstract
Abstract The lactate dehydrogenase (LDH) isoenzyme distribution was measured in T γ + lymphocytes from normal individuals. T γ + lymphocytes were obtained from purified T lymphocytes by ox-IgG rosette sedimentation. The B:A subunit ratio was clearly lower in the T γ + lymphocytes. Phenotyping of the T γ + lymphocytes showed a vast majority of OKT11 + , OKM1 + , OKT3 − lymphocytes. In one experiment, T γ + lymphocytes were enriched by OKT3 depletion of T lymphocytes. The low B:A ratio was also found in these cells indicating that the LDH pattern is not the consequence of an in vitro activation by immune complexes. As the T γ + lymphocytes were considerably enriched in cells having the characteristics of NK cells according to their phenotyping, morphology and NK cell activity, we may assume that NK cells have a low B:A ratio.
Authors
J. Plum, M. Smedt, L. Sabbe
Journal
Immunology Letters