Finding
SDF-1 can improve the proliferation and migration abilities of CSCs by SDF-1.
Paper
Mechanism investigation on improving proliferation and migration abilities of cardiac stem cells by stromal cell derived factor-1
In Vitro Trial
Citations: 0
Full textAbstract
Objective:To investigate the mechanism on improving proliferation and migration abilities of cardiac stem cells(CSCs)by stromal cell derived factor-1(SDF-1).Methods:CSCs were isolated from adult mice hearts purified by magnetic-activated c-kit cell sorting magnetic beads.The markers of c-kit and Sca-1 were measured by FACS.The cells were cultured with SDF-1 and CXCR4-selective antagonist AMD3100,and c-kit expression was measured by qPCR and Western blotting.After the intervention of SDF-1 and AMD3100,proliferation and migration of c-kit( +) CSCs were measured by CCK-8 assay and transwell assay.Results:More than 80%cardiosphere-derived cells which were purified by magnetic-activated c-kit cell sorting magnetic beads were positive for c-kit and Sca-1 by FACS.SDF-1 could enhance the expression of c-kit mRNA and protein,and AMD3100could inhibit this function.And SDF-1 could enhance the proliferation and migration of c-kit( +) CSCs,and AMD3100 could inhibit these functions.Conclusion:SDF-1 can improve the proliferation and migration abilities of CSCs by SDF-1.
Authors
Chen Zhong-p
Journal
Journal of Southeast University