We used gel-permeation high-performance liquid chromatography to detect cytosolic and nuclear calcitriol (1,25-dihydroxycholecalciferol) receptors. This new convenient technique allowed accurate separation of the calcitriol receptor from other vitamin D-binding proteins. Compared with sucrose-density-gradient centrifugation, it has several advantages including, in particular, its rapidity.
J. Danan, H. Mathieu
The Biochemical journal