We collected centric diatom ”Stephanopyxis palmeriana” samples in coastal waters of Xiamen for characteristic red light/far red light (R/FR) phytochrome reactions to identify its photoreceptor in the course of sexual reproduction. The result showed that pre-illumination of 2-3h red light before darkness could induce sexualization of ”S. palmeriana”, while the follow-up illumination of far red light could reverse the effect of red light, which is a featured reaction of phytochrome. The Southern Dot Blot was carried out to identify the type of phytochrome that induces the sexualization. The result also showed high homogeneity of DNA fragment of ”S. palmeriana” with phyB, but phyA. This means the photoreceptor in the process of sexual reproduction of ”S. palmeriana” is phytochrome B (phyB).
R. Hu, Junmin Lin, Qiuqi Lin
Chinese Journal of Oceanology and Limnology