2D-DIGE in proteomics applications

2D-DIGE in proteomics applications

Published 2011 · Du Jun-bian

Journal of Biology

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Abstract

Two-way fluorescence difference gel electrophoresis(2D-DIGE) as one of new analytical techniques was widely used in study of animals,plants,microbes and proteins of human differences.In animal medicine,using DIGE technology,through different types of cells in different individuals,organizations,or through different treatments and different protein expression under different growth conditions analyzed in the study of molecular mechanisms of disease,molecular diagnostics,drug mechanism,drug science has a wide range of application areas.In botany,the technology can be used to isolate and analyze the subcellular structures of plant proteins as well as in biotic and abiotic stress,plant cells differences in protein expression in order to establish differences in protein interaction network graph,and thus to study the damage mechanism to provide some basis.

Study Snapshot

Key takeaway2D-DIGE technology is a versatile tool for studying protein expression differences in various organisms, aiding in molecular diagnostics, drug discovery, and plant research.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

2D-DIGE in proteomics applications

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