Effect of 4-nonylphenol on cell proliferation and adipocyte formation in cultures of fully differentiated 3T3-L1 cells.

doi:10.1093/TOXSCI/KFG203

Paper

Effect of 4-nonylphenol on cell proliferation and adipocyte formation in cultures of fully differentiated 3T3-L1 cells.

Published Oct 1, 2003 · H. Masuno, Shoko Okamoto, J. Iwanami

Toxicological sciences : an official journal of the Society of Toxicology

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57

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Abstract

The effect of 4-nonylphenol (NP) on cell proliferation and adipocyte formation was examined in cultures of fully differentiated 3T3-L1 cells. Following the hormonal induction of differentiation into adipocytes, 3T3-L1 cells were treated for 8 days with or without NP. NP at 5 and 10 microg/ml increased the DNA content by 32% and 68%, respectively, compared with that of the untreated cultures, in which NP was absent during the treatment period. There were many more bromodeoxyuridine (BrdU)-positive cells in the NP-treated cultures, in which NP was present at a concentration of 10 microg/ml during the treatment period, compared to the untreated cultures. These results indicate that NP had the ability to stimulate the proliferation of fully differentiated 3T3-L1 cells. NP at 5 and 10 microg/ml decreased the triacylglycerol (TG) content by 26% and 58%, respectively, and decreased the lipoprotein lipase (LPL) activity by 51% and 71%, respectively. The lipid droplets in individual cells of the NP-treated cultures were smaller than those of the untreated cultures. The mRNA levels of LPL and adipocyte-specific fatty acid binding protein (aP2) were considerably lower in the NP-treated cultures than in the untreated cultures. Thus, NP also had the ability to inhibit adipocyte formation in cultures of fully differentiated 3T3-L1 cells. A study using an antiestrogen ICI 182,780 showed that the NP-stimulated cell proliferation was mediated partly by the estrogen receptor, while the NP-induced inhibition of adipocyte formation was mediated by a mechanism other than the estrogen receptor.

In Vitro Study

Study Snapshot

4-nonylphenol stimulates cell proliferation and inhibits adipocyte formation in fully differentiated 3T3-L1 cells, with its effects partly mediated by the estrogen receptor and other mechanisms.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Paper

Effect of 4-nonylphenol on cell proliferation and adipocyte formation in cultures of fully differentiated 3T3-L1 cells.

References

Bisphenol A in combination with insulin can accelerate the conversion of 3T3-L1 fibroblasts to adipocytes.

BPA alone can trigger adipocyte differentiation, but when combined with insulin, it accelerates the process.

Reproductive effects of nonylphenol in rats after gavage administration: a two-generation study.

Nonylphenol has no observed adverse effect on reproductive capacity in rats, with the NOAEL being 50 mg/kg/day or greater in parent animals and 10 mg/kg/day in the next generation.

Formation of hydroxy radicals by environmental estrogen-like chemicals in rat striatum

Environmental estrogen-like chemicals, para-nonylphenol and bisphenol A, stimulate hydroxy radical formation in rat striatum, with tamoxifen effectively inhibiting this effect.

4-Nonylphenols and 4-tert-octylphenol in water and fish from rivers flowing into Lake Biwa.

4-nonylphenols (NOs) are present in river water year-round, with minimum concentrations at 5-8°C in winter, and 4-tert-octylphenol (OC) is found in fish at lower concentrations and lower frequencies.

Estradiol Stimulation of c-fos and c-jun Expressions and Activator Protein-1 Deoxyribonucleic Acid Binding Activity in Rat White Adipocyte.

Estradiol stimulates c-fos and c-jun expression in female rats' fat cells through induction of the AP-1 complex and modulation of AP-1 dependent gene expression in adipocytes.

Citations

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