8-Iso-PGF(2alpha) administration generates dysmorphogenesis and increased lipid peroxidation in rat embryos in vitro.

doi:10.1002/TERA.10068

Paper

8-Iso-PGF(2alpha) administration generates dysmorphogenesis and increased lipid peroxidation in rat embryos in vitro.

Published Oct 1, 2002 · P. Wentzel, U. Eriksson

Teratology

UNKNOWN SJR score

30

Citations

3

Influential Citations

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Abstract

BACKGROUND Diabetic pregnancy displays increased incidence of congenital malformations and elevated levels of lipid peroxides in the offspring. The aim of the present work was to study if exogenous administration of one lipid peroxide, the isoprostane 8-iso-PGF(2alpha), is teratogenic per se in rat embryos in vitro, and if such teratological effects may be diminished by supplementation of an antioxidative agent, i.e., N-acetylcysteine or superoxide dismutase, to the culture medium. METHODS Day-9 embryos were cultured in vitro for 48 hr and subjected to 8-iso-PGF(2alpha) with and without N-acetylcysteine or superoxide dismutase. RESULTS Addition of 2 micromol/l of the isoprostane 8-iso-PGF(2alpha) to the culture medium caused high malformation rate, decreased protein and DNA contents, decreased somite number and crown-rump-length as well as marked accumulation of the isoprostane in the embryonic tissues. Adding N-acetylcysteine or superoxide dismutase to the culture medium with isoprostane normalized almost all morphological and biochemical parameters, including the elevated tissue concentration of 8-iso-PGF(2alpha). CONCLUSIONS Results indicate that the isoprostane (8-iso-PGF(2alpha)) serves both as an oxidative stress indicator and a teratogenic agent. The findings support earlier studies of enhanced oxidative stress and increased malformation rate in embryos exposed to a diabetes-like environment, and suggest prevention of dysmorphogenesis by administration of antioxidative agents.

In Vitro Study

Study Snapshot

Isoprostane (8-iso-PGF(2alpha)) serves as both an oxidative stress indicator and teratogenic agent in rat embryos, and its dysmorphogenesis can be prevented by adding antioxidative agents like N-acetylcysteine or superoxide

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Paper

8-Iso-PGF(2alpha) administration generates dysmorphogenesis and increased lipid peroxidation in rat embryos in vitro.

References

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Citations

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Targeting glucose and phospholipid metabolism in early pregnancy may help prevent birth defects by addressing key processes and factors in glucose and phospholipid metabolism.

Therapeutic interventions

Maternal hyperglycemia leads to embryonic malformations, and developing multi-nutrient dietary supplements may help prevent these abnormalities.

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