G. Tesoriere, R. Vento, D. Magistro
Mar 1, 1971
European journal of biochemistry
In this paper the metabolism of amino sugars in the retina and in the pigment epithelial cells has been studied. Extracts obtained from the retina and from the pigment epithelial cells are capable, in the presence of suitable cofactors, of converting [1-14C]glucosamine into UDP-N-acetylhexosamines. Chromatographic investigations with the use of Dowex resin column have permitted the identification of the principal intermediaries of amino sugar metabolism: GlcNAc, GlcN-6-P, GlcNAc-6-P, UDP-GlcNAc, UDP-GalNAc. Both the retina and the pigment epithelial cells are, further, capable of synthesizing those amounts of acetyl coenzyme A necessary for the formation of acetylated amino sugars. The metabolism of amino sugars in the retina would appear to be much more active than in the pigment epithelial cells; this would indicate that the greater part of the synthesis of mucopoly-saccharides of the interstitial matrix takes place in the retina. In the course of this research, furthermore, the presence of three compounds has been ascertained, the identification of which is still in progress.