H. Shiba, K. Hinata, A. Suzuki
Journal name not available for this finding
The self incompatibility (SI) system of Brassica is sporophytically controlled by multiallenic genes at an S-locus. Two genes, SLG (S-locus glycoprotein) and SRK(S-receptor protein kinase), on the S-locus are thought to play an important role in recognition of the self pollen, though there is no direct evidence. We introduced the antisense SLG gene to self incompatible Brassica species through Agrobacterium-mediated transformation. We found that a transgenic plant of B. raga (syn. B. campestris) could set seeds after self pollination, and that no SLG protein was detectable in a gel blot analysis of the transgenic plant. These observations provide strong evidence that SLG and/or SRK are directly involved in the pollen-stigma recognition of SI.