Changes in luminal plasma and disappearance of spermatozoa from the ligated cauda epididymidis of the androgen-deficient rabbit.
R. Jones, H. Dott
Sep 1, 1980
Citations
19
Citations
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Journal
Journal of reproduction and fertility
Full text
Semantic Scholar
Key Takeaway Key Takeaway: Castration in androgen-deficient rabbits leads to the disappearance of most spermatozoa within 5 weeks, with testosterone administration preventing these changes in luminal plasma composition.
Abstract
By 3 weeks after castration the majority of spermatozoa in the ligated cauda epididymidis were dead and decapitated and there was a 46% reduction in the total number of spermatozoa. By 5 weeks after castration > 99% of spermatozoa had disappeared. Electrophoresis of cauda epididymal plasma (CEP) on polyacrylamide gels revealed that after castration the protein composition of CEP changed from a pattern specific to the epididymis to one similar to blood serum. These changes in the luminal content could be prevented by administering testosterone. When spermatoza from the cauda epididymidis were diluted in CEP from normal rabbits they remained motile for several hours at room temperature. Dilution in CEP from 3- ro 5-week castrated animals stimulated vigorous forward motility but this declined to very low levels after 40 min. The evidence indicates that dead spermatozoa can be removed from the epididymis after castration by mechanisms not involving granuloma formation.