Conductance stopped-flow study of the association reactions of anionic colloidal spheres with cationic spheres and with bovine serum albumin molecules
T. Okubo, H. Kitano, Satoshi Iwai
1988
Citations
2
Citations
Journal
Journal of the Chemical Society, Faraday Transactions
Full text
Semantic Scholar
Key Takeaway Key Takeaway: The association reactions of anionic spheres with cationic spheres and bovine serum albumin show small forward rate constants, with sphere size increasing the association constant and pH decreasing the rate constant.
Abstract
The equilibrium and kinetic parameters of the association reactions of anionic spheres with cationic spheres and with bovine serum albumin (BSA) are evaluated using the conductance stopped-flow technique. The forward rate constants (kf) of the binary association between spheres of opposite charge are close to the theoretical values for the diffusion-controlled association process of neutral species. The kf values of association between the colloidal spheres and BSA molecules are much smaller than those corresponding to diffusion-controlled rates. kf and kb decrease and the association constant (kf/kb) increases as the sphere size increases. kf increases sharply, whereas kb remains constant as the pH values of the mixtures decrease. These kinetic features are discussed.