Jian-ping Wang, Shu-jin Zhao, Jie Wang
Nov 1, 2004
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
AIM To construct phage display library of anti-chymopapain scFv. METHODS V(H) and V(L) gene repertoires were amplified from splenocyte mRNA by RT-PCR and joined by a (Gly(4)ser)3 linker to obtain scFv genes. The scFv genes were then cloned into phagemid pFAB5C to construct phage display library. Affinity selection and ELISA were used to identify specific phage antibody to chymopapain. RESULTS After 4 rounds of panning, high affinity scFv was obtained. CONCLUSION Phage display library of anti-chymopapain scFv was successfully constructed, and scFv with binding ability to chymopapain was obtained.