The in vitro culture of embryonic chicken heart cells.
H. Schneider, M. Shaw, E. E. Muirhead
Sep 1, 1965
Citations
18
Citations
Journal
Experimental cell research
Full text
Semantic Scholar
Key Takeaway Key Takeaway: Successful in vitro culture of embryonic chicken heart cells has been achieved using modified mammalian cell culture techniques and methylcellulose-enriched trypsin solution.
Abstract
Abstract Modifications of mammalian cell culture technic have resulted in the successful development of embryonic chicken heart cell strains. These include (1) the addition of methylcellulose to the trypsin solution, (2) unfiltered fetal bovine serum in the nutrient medium and (3) the use of a disposable plastic hood to prevent cross-contamination with other cell lines. These cells have been successfully subcultured 41 times and recovered after storage at −170 °C.
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