H. Schneider, M. Shaw, E. E. Muirhead
Sep 1, 1965
Experimental cell research
Abstract Modifications of mammalian cell culture technic have resulted in the successful development of embryonic chicken heart cell strains. These include (1) the addition of methylcellulose to the trypsin solution, (2) unfiltered fetal bovine serum in the nutrient medium and (3) the use of a disposable plastic hood to prevent cross-contamination with other cell lines. These cells have been successfully subcultured 41 times and recovered after storage at −170 °C.