Paper
Cytotoxicity of 1-octyl-3-methylimidazolium chloride on Escherichia coli DH5α
Published Aug 4, 2014 · C. Jing, Huanhuan Hu, Menglong Guo
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Abstract
Abstract The mechanisms of the cytotoxic effect of ionic liquid (IL) 1-octyl-3-methylimidazolium chloride ([C8mim][Cl]) on bacterial cells were studied. Bacterial cells were exposed to 0, 0.1, 1 and 10 mmol ċ L−1 [C8mim][Cl] for 10 h. The results showed that [C8mim][Cl] inhibited bacterial cell proliferation in a dose-dependent manner. When IL concentrations reached 10 mmolċL−1, almost no cell division was observed. [C8mim][Cl] treatment significantly reduced succinate dehydrogenase activity. The amount of total protein significantly decreased with increasing [C8mim][Cl] concentrations of up to 10 mmolċL−1. [C8mim][Cl] increased protein and nucleic acid contents in the extracellular fluid and increased cell permeability. The results suggest that the increase in cell membrane permeability, which was induced by [C8mim][Cl], could inhibit cell proliferation and metabolic activity in bacteria.
The ionic liquid 1-octyl-3-methylimidazolium chloride inhibits bacterial cell proliferation and metabolic activity by increasing cell permeability and reducing protein levels.
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