The effect of the fluorescent probe, 3,3'-dipropylthiodicarbocyanine iodide, on the membrane potential of Ehrlich ascites tumor cells.

doi:10.1016/0006-291X(80)90845-1

Paper

DOI: 10.1016/0006-291X(80)90845-1

The effect of the fluorescent probe, 3,3'-dipropylthiodicarbocyanine iodide, on the membrane potential of Ehrlich ascites tumor cells.

Published Jul 31, 1980 · T. C. Smith, S. Robinson

Biochemical and biophysical research communications

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Abstract

Abstract hidden due to publisher request; this does not indicate any issues with the research. Click the full text link above to read the abstract and view the original source.

In Vitro Study

Study Snapshot

The fluorescent dye 3,3′-dipropylthiodicarbocyanine iodide induces membrane hyperpolarization and increases permeability to K+ by 65% in Ehrlich ascites tumor cells.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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References

Citations

DOI: 10.1007/BF01871776

Membrane potential, anion and cation conductances in Ehrlich ascites tumor cell

Ehrlich ascites tumor cells show a depolarization of membrane potential due to potassium and sodium diffusion, and a decrease in cation permeability after treatment with cation ionophores and hypotonic cell swelling.

1989·37citations·I. H. Lambert et al.·The Journal of Membrane Biology

The Journal of Membrane Biology

DOI: 10.1007/BF00585065

Electrical properties of Ehrlich ascites tumor cells

Ehrlich ascites tumor cells have a cell membrane potential similar to other mammalian epithelial cells, mainly driven by potassium diffusion, with small conductances to sodium and chloride.

1987·4citations·E. Gstrein et al.·Pflügers Archiv

Pflügers Archiv

DOI: 10.1016/0005-2736(86)90304-4

Differential effects of transmembrane potential on two Na+-dependent transport systems for neutral amino acids.

Hyperpolarization of membrane potential increases amino acid uptake through Na+-dependent system A, while system ASC and L are insensitive to membrane potential changes.

1986·18citations·M. Valdeolmillos et al.·Biochimica et biophysica acta

Biochimica et biophysica acta

DOI: 10.1016/0304-4165(85)90044-3

Effects of probes of membrane potential on metabolism in synaptosomes.

TPP + and rhodamine 6G probes for measuring membrane potential have negligible effects on metabolism in synaptosomes, while diS-C 3-(5) significantly inhibits respiration.

1985·26citations·T. Aiuchi et al.·Biochimica et biophysica acta

Biochimica et biophysica acta

DOI: 10.1002/jcp.1041230306

Oxonol dyes as monitors of membrane potential: The effect of viruses and toxins on the plasma membrane potential of animal cells in monolayer culture and in suspension

Oxonol dyes effectively monitor plasma membrane potential in animal cells, revealing depolarization and cation leakage in cells infected with certain viruses.

1985·49citations·C. Bashford et al.·Journal of Cellular Physiology

Journal of Cellular Physiology

DOI: 10.1016/0167-4889(83)90063-0

Reduction in accumulation of [3H]triphenylmethylphosphonium cation in neuroblastoma cells caused by optical probes of membrane potential. Evidence for interactions between carbocyanine dyes and lipophilic anions.

Carbocyanine dyes reduce the accumulation of [3H]triphenylmethylphosphonium cation in neuroblastoma cells due to interactions with tetraphenylboron, not depolarizing the plasma membrane.

1983·5citations·G. Milligan et al.·Biochimica et biophysica acta

Biochimica et biophysica acta

DOI: 10.1016/0005-2736(82)90439-4

Measurement of membrane potential in polymorphonuclear leukocytes and its changes during surface stimulation.

Membrane depolarization in polymorphonuclear leukocytes is not the immediate stimulus for the respiratory burst, as it occurs in the absence of extracellular sodium.

1982·43citations·M. Kuroki et al.·Biochimica et biophysica acta

Biochimica et biophysica acta

DOI: 10.1016/S0006-291X(82)80076-4

A carbocyanine dye, DiOC6(3), acts as a mitochondrial probe in human neutrophils.

DiOC6(3) is a mitochondrial probe in human neutrophils, predominantly reporting changes in mitochondrial membrane potential rather than transplasmalemmal membrane potential.

1982·81citations·H. Korchak et al.·Biochemical and biophysical research communications

Biochemical and biophysical research communications

DOI: 10.1002/jcp.1041120222

The use of flurescent dyes to measure membrane potentials: A response

Fluorescent cyanine dyes for measuring membrane potential in cell suspensions may cause alteration, energy metabolism effects, and inaccurate fluorescence calibration, making their use misleading.

1982·30citations·Thomas C. Smith·Journal of Cellular Physiology

Journal of Cellular Physiology