Chinese Journal of Pathophysiology
AIM: To explore the role of miRNA expression in the process of antitumor drug demethylcantharidin(DMC)-induced leukemia cell apoptosis. METHODS: K562 cells were treated with DMC to induce apoptosis.Microarray assessment was performed to detect the changes of miRNAs.The expression of miRNAs was further detected by RT-PCR and real-time PCR.The miRNA-relative genes were analyzed by gene information softwares,and their expression was determined by ELISA analysis. RESULTS: The results of microarray showed that more than 290 miRNAs were differentially expressed after DMC treatment.The expression of miR-16,miR-34 and miR-125 increased at more than 1.5 folds in DMC treatment group determined by RT-PCR and real-time PCR analysis,while both miR-106 and miR-150 were down-expressed over 60%.Using microRNA TargetScan and miRanda analysis software,we found that the expression of oncogenes(bcl-2,E2F1,E2F3) and tumor suppressor genes(RB1,p53) may be regulated by the above miRNAs.The expression of RB1 and P53 proteins significantly increased,while Bcl-2,E2F1 and E2F3 proteins were obviously down-regulated after DMC treatment. CONCLUSION: DMC induces K562 cell apoptosis by regulating the expression of miRNAs and their relative genes.