Paper
Effect of Modified Heptamethyltrisiloxane on the Efficiency of Agrobacterium-Mediated Transformation and Expression of Recombinant Structure in Plant Cell and Tissue Culture
Published Feb 1, 2016 · M. Skaptsov, M. Kutsev, M. Filipenko
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Abstract
Detergents represent a unique class of chemical compounds. They can alter surface and interphase bonds, and form micellar systems. These detergent properties allow to alter wettability of surfaces, stabilize or destabilize dispersed systems, and modify the properties of liquid phases. Therefore, the use of detergents is virtually unlimited in chemical synthesis and processing, medicine, biological systems and agricultural biology. The article includes the studies of the feasibility of application of 1,1,1,3,5,5,5-heptamethyltrisiloxane modified by polyalkylene oxide in combination with allyloxypolyethyleneglycol in the ratio of 10:1 as a detergent for agrobacterial-mediated transformation. Tween 20 detergent was used as a means of control in the concentration of 5%. As a result of histochemical analysis of transformed tissues, a significant difference was determined in expression of beta-glucuronidase reporter gene. The study of gene expression by calculating the relative content of mRNA showed that the initial number of mRNA copies of genetic makers transfected by co-culturing in a liquid medium with addition of Tween 20 is on the average 32% lower than when using modified heptamethyltrisiloxane
Modified heptamethyltrisiloxane significantly improves the efficiency of agrobacterial-mediated transformation and recombinant gene expression in plant cell and tissue culture compared to Tween 20.
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