Effects of 4-nitroquinoline-N-oxide on RNA synthesis.

Paper

Effects of 4-nitroquinoline-N-oxide on RNA synthesis.

Published Mar 1, 1969 · J. Paul, R. Reynolds, P. Montgomery

Cancer research

Q1 SJR score

7

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0

Influential Citations

Semantic Scholar

Abstract

Summary 4-Nitroquinoline- N -oxide, a potent mutagenic, carcinogenic, and carcinostatic agent, has been studied with respect to its effects on RNA synthesis using sucrose gradients, methylated albumin Kieselguhr columns, and the electron microscope. An analysis of polyribosomal amino acid incorporation in tissue culture systems exposed to 4-nitroquinoline- N -oxide demonstrated a marked inhibition of the incorporation of amino acids by polyribosomal structures; the inhibition of amino acid incorporation was apparent within one hour after exposure to 4-nitroquinoline- N -oxide. A few hours after the addition of 4-nitroquinoline- N -oxide, increased amounts of low molecular weight RNA could be demonstrated by trichloracetic acid extraction or methylated albumin Kieselguhr chromatography. This increase probably represented active synthetic products and was not derived primarily from degraded RNA of higher molecular weight. Exposure of HeLa cells to 4-nitroquinoline- N -oxide for 24 hours resulted in depressed synthesis in all RNA fractions; RNA sedimenting below 10 S was less depressed than heavier RNA fractions, as evaluated by sucrose density gradient centrifugation. The similarity between RNA synthesis in the presence of 4-nitroquinoline- N -oxide and actinomycin D in low dosages is discussed, and the possible similarities in the sites of action are described.

In Vitro Study

Study Snapshot

4-nitroquinoline-N-oxide inhibits amino acid incorporation in polyribosomal structures, leading to increased low molecular weight RNA and depressed RNA synthesis in cells.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Paper

Effects of 4-nitroquinoline-N-oxide on RNA synthesis.

References

Differential inhibition by 4-nitroquinoline-N oxide in serially propagated mammalian cell lines.

NQO inhibition affects mammalian cell lines differently, with HeLa cells being most susceptible and ChL and RNb cells showing similar inhibition effects.

Interaction of the Water-Soluble Carcinogen 4-Nitroquinoline N-Oxide with DNA

4-nitroquinoline N-oxide interacts with native DNA, suggesting its carcinogenicity, and may be related to its interaction with RNA and other nucleic acid derivatives.

RNA metabolism in the HeLa cell nucleus.

The nuclear fraction of HeLa cells contains early precursors to ribosomal RNA, and the cleavage of 45 s RNA to 16 s and 35 s RNA leads to the formation of ribosomal RNA.

Interaction of polynuclear aromatic hydrocarbons, 4‐nitroquinoline 1‐oxides, and various dyes with DNA

Polynuclear aromatic hydrocarbons, 4nitroquinoline 1oxides, and various dyes show specific interactions with DNA, with purines being active sites of interaction.

Subribosomal particles and the transport of messenger RNA in HeLa cells.

The 45 s subribosomal particle may play a role in the assembly of polysomes and transporting messenger RNA from nucleus to cytoplasm in HeLa cells.

NUCLEOLAR "CAPS" PRODUCED BY ACTINOMYCIN D.

Actinomycin D and 4-nitroquinoline N-oxide both cause specific morphologic changes in nucleoli and nuclei, suggesting they may affect DNA-dependent RNA synthesis.

Citations

Two-dimensional titanium carbide (MXene) nanosheets as an efficient electrocatalyst for 4-nitroquinoline N-oxide detection

Two-dimensional titanium carbide (MXene) nanosheets provide efficient electrochemical performance for 4-nitroquinoline N-oxide detection, with a wide linear range and low limit of detection (2 nM).

Synthesis of Quinoline N-Oxides by Cobalt-Catalyzed Annulation of Arylnitrones and Alkynes

This study presents a new, straightforward method for synthesizing quinoline N-oxides from arylnitrones and alkynes, enabling the synthesis of quinoline N-oxides under mild conditions.

2-(4-Methylphenyl)-1-phenylsulfonyl-3-nitro-1,2-dihydroquinoline

The crystal structure of 2-(4-Methylphenyl)-1-phenylsulfonyl-3-nitro-1,2-dihydroquinoline shows a zigzag chain along the [101] direction, with weak intermolecular C-HO interactions linking molecules into

Complexation of some phenol hosts with quinoline N-oxide and the structural studies of their complexes by X-ray crystallography

The 1:1 inclusion complexes of 4,4'-isopropylidenebisphenol, 4,4'-cyclohexylidenebisphenol, and 4-(1,1,3,3-tetramethylbutyl)phenol with quinoline N-oxide show good yields and molecular arrangements

Carcinogenesis in tissue culture. XIII. Binding of 4‐nitroquinoline 1‐oxide‐3h to nucleic acids and proteins of l.P3 and JTC‐25.P3 cells

4NQO carcinogen inhibits growth in L.P3 and JTC-25.P3 cells, with higher incorporation in lower population densities, and its binding to macromolecular constituents persists for several hours.