The formation of 9-hydroxychrysene-1,2-diol as an intermediate in the metabolic activation of chrysene.

doi:10.1093/CARCIN/6.1.135

Paper

The formation of 9-hydroxychrysene-1,2-diol as an intermediate in the metabolic activation of chrysene.

Published 1985 · R. Hodgson, A. Seidel, W. Bochnitschek

Carcinogenesis

Q1 SJR score

18

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0

Influential Citations

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Abstract

9-Hydroxy-trans-1,2-dihydro-1,2-dihydroxychrysene (9-hydroxychrysene-1,2-diol), which may be the triol involved in the formation of a chrysene triol-epoxide-DNA adduct in mouse skin, was not detected when chrysene was incubated with rat-liver microsomal preparations. In separate experiments an excess of synthetic 9-hydroxychrysene-1,2-diol was added during the incubation of 3H-labelled chrysene with rat-liver microsomes and was then re-isolated. The triol was found to contain a radioactive product that had chromatographic properties identical to those of 9-hydroxychrysene-1,2-diol when examined by reverse-phase h.p.l.c., both before and after acetylation, by normal-phase h.p.l.c. and by t.l.c. both before and after oxidation. When treated with m-chloroperoxybenzoic acid, the synthetic 9-hydroxychrysene-1,2-diol formed products that possessed alkylating activity and that reacted with DNA in vitro. Examination of the triol-epoxides produced by oxidation of a mixture of synthetic and metabolic 9-hydroxychrysene-1,2-diol by t.l.c. suggested that the anti-isomer was formed.

In Vitro Study

Study Snapshot

The formation of 9-hydroxychrysene-1,2-diol as an intermediate in the metabolic activation of chrysene may be involved in the formation of chrysene triol-epoxide-DNA adducts in mouse skin.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

The formation of 9-hydroxychrysene-1,2-diol as an intermediate in the metabolic activation of chrysene.

References

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