Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells

doi:10.1007/s11655-010-0033-z

Paper

Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells

Published Feb 4, 2010 · Xiaoying Wang, Xiumei Gao, Hong Liu

Chinese Journal of Integrative Medicine

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15

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0

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Abstract

ObjectivePeriplocin is an active digitalis-like component from Cortex Periplocae, which has been widely used in the treatment of heart diseases in China for many years. According to the recommendations on the cardiovascular effect of periplocin from in vivo experiments, subsequent in vitro experiments are greatly needed for the global assessment of periplocin. The objective of this study is to investigate the cell proliferation effect and the mechanism of periplocin on endothelial cells.MethodsThe proliferative activity of periplocin (0.4, 2, 10, 50, 250 μmol/L; 6, 12, 24, 48, 72 h) was investigated by a comparison with the well-reported cardiac glycoside, ouabain, on mouse cardiac microvascular endothelial cells (CMEC). 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH) and 5-bromo-2-deoxyuridine (BrdU) assays were used to evaluate cell proliferation and viability. Subsequently, cDNA microarray experiments were performed on periplocin- (50 μmol/L) and ouabain- (50 μmol/L) treated cells, and data was analyzed by ArrayTrack software.ResultsPeriplocin could increase cell viability to a level lower than ouabain in the MTT analysis, but decrease LDH release simultaneously. The BrdU incorporation assay showed an increase in cell proliferation with 2–50 μmol/L periplocin. Genes related to protein serine/threonine kinase were the most significantly enriched in the 160 genes identified in periplocin versus the control. In the 165 genes regulated by periplocin versus ouabain, GTP-binding was the most altered term.ConclusionsThe results demonstrated the proliferation action of periplocin on CMEC. Meanwhile, its lower cytotoxicity compared to ouabain provides a new insight into the treatment of heart failure.

In Vitro Study

Study Snapshot

Periplocin increases cell proliferation and has lower cytotoxicity compared to ouabain, offering potential for heart failure treatment.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Gene expression profiling of the proliferative effect of periplocin on mouse cardiac microvascular endothelial cells

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