Inducible gene expression system by 3-hydroxypropionic acid

doi:10.1186/s13068-015-0353-5

Paper

Inducible gene expression system by 3-hydroxypropionic acid

Published Oct 20, 2015 · Shengfang Zhou, Satish Kumar Ainala, E. Seol

Biotechnology for Biofuels

Q1 SJR score

32

Citations

2

Influential Citations

Full textSemantic Scholar

Abstract

Background3-Hydroxypropionic acid (3-HP) is an important platform chemical that boasts a variety of industrial applications. Gene expression systems inducible by 3-HP, if available, are of great utility for optimization of the pathways of 3-HP production and excretion.ResultsHere we report the presence of unique inducible gene expression systems in Pseudomonas denitrificans and other microorganisms. In P. denitrificans, transcription of three genes (hpdH, mmsA and hbdH-4) involved in 3-HP degradation was upregulated by 3-HP by the action of a transcriptional regulator protein, LysR, and a cis-acting regulatory site for LysR binding. Similar inducible systems having an LysR transcriptional regulator were identified in other microorganisms that also could degrade 3-HP. A docking study showed that the 3-HP binding pocket is located between the N-terminal helix-turn-helix motif and the C-terminal cofactor-binding domain.ConclusionsThis LysR-regulated 3-HP-inducible system should prove useful for control of the level of gene expression in response to 3-HP.

Study Snapshot

This LysR-regulated 3-hydroxypropionic acid-inducible system in Pseudomonas denitrificans and other microorganisms could be useful for controlling gene expression levels in response to 3-HP, potentially improving industrial applications.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Inducible gene expression system by 3-hydroxypropionic acid

References

Citations