Paper
Hydantocidin: a possible proherbicide inhibiting purine biosynthesis at the site of adenylosuccinate synthetase
Published Oct 1, 1995 · D. Heim, C. Cseke, B. Gerwick
Pesticide Biochemistry and Physiology
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Abstract
Abstract Hydantocidin is a highly active phytotoxin that causes immediate cessation of plant growth, meristematic necrosis, and plant death. Injury symptoms and metabolite reversal studies in Arabidopsis thaliana suggested a novel mode of action, most likely in purine biosynthesis. Adenine supplementation alleviated symptoms associated with hydantocidin, while the addition of guanine had no effect. Adenine reversal resulted from the in vivo conversion of adenine to adenosine 5′-phosphate. Hydantocidin also inhibited the incorporation of radiolabeled glycine into adenine nucleotides, but not into guanine nucleotides. Adenosine 5′-triphosphate levels in Arabidopsis were shown to drop dramatically after treatment with hydantocidin. All of the aforementioned physiological effects were also elicited by hadacidin, a known inhibitor of the conversion of inosine 5′-phosphate to adenosine 5′-phosphate, at the site of adenylosuccinate synthetase. However, hy dantocidin was subsequently shown not to be an in vitro inhibitor of either adenylosuccinate synthetase or adenylosuccinate lyase (the two enzymes that mediate the conversion of inosine 5′-phosphate to adenosine 5′-phosphate). We hypothesized that hydantocidin might be converted in vivo to another molecule that inhibits the formation of adenosine 5′-phosphate. This hypothesis was supported when increasing amounts of an adenylosuccinate synthetase inhibitor were formed in a plant extract, over time, after adding hydantocidin, ATP, and MgCl 2 .
Hydantocidin may be a potential proherbicide that inhibits purine biosynthesis, causing plant growth cessation and necrosis, and may be converted in vivo to an inhibitor of adenosine 5′-phosphate formation.
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