Aman Kumar, S. Maan, A. Ghosh
Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Identification of orf virus (ORFV) from an outbreak of contagious pustular dermatitis (CPD) in flocks of goats, at the Central sheep breeding farm (CSBF), Hisar, Haryana is reported. Out of 89 bucks and 175 female hoggets, 28 bucks and 38 female hoggets were infected with severe proliferative scabby lesions on the muzzle, lips, oral mucosa and limbs to a variable extent. The viral DNA was isolated from scabs and swab suspensions and further identified using B2L gene specific set of primers in PCR and sequencing. Phylogenetic analysis revealed that the virus showed high nucleotide identity with previously reported Indian isolates. This report described the reliable use of molecular tools for accurate and confirmatory diagnosis of ORFV infection.