Identification of genes encoding T cell defined tum- antigens.

Paper

Identification of genes encoding T cell defined tum- antigens.

Published Jan 1, 1989 · B. Lethé, E. de Plaen, C. Lemoine

Princess Takamatsu symposia

3

Citations

0

Influential Citations

Semantic Scholar

Abstract

Tum- mutants are immunogenic mutants obtained by mutagen treatment of mouse tumor cells. They express new "tum- antigens" recognized by cytolytic T cells (CTL) but not by specific antibodies. We have recently developed a method aimed at cloning the genes coding for such transplantation antigens. It is based on gene transfection and detection of transfectants by their ability to stimulate CTL. Tum- gene P91A has been isolated. It codes for a 60 kDa protein which does not carry a signal sequence at its N-terminus. The tum- allele differs from its normal counterpart by a point mutation. The sequence of this gene and that of two other tum- genes are totally unrelated with each other and with any sequence presently recorded in data banks. We will try to apply the same cloning method to isolate mouse and human tumor-specific transplantation antigens (TSTA).

Study Snapshot

This study successfully cloned tum- gene P91A, a new immunogenic antigen in mouse tumor cells, using a novel method to identify tumor-specific transplantation antigens.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Identification of genes encoding T cell defined tum- antigens.

References

Citations