D. Raharjo, R. Yulistiani, W. Setyarini
Oct 8, 2019
IOP Conference Series: Materials Science and Engineering
Rapid diagnosis of pathogenic bacteria in food is closely related to safety. We modified a Culture-based methods for Salmonella sp. which rapidly identified Salmonella enterica serovars Typhi and Salmonella enterica serovars Paratyphi A from chicken meat samples. The World Health Organization method consists of six steps of the test, sequentially pre-enrichment, selective enrichment, selective diagnostic isolation, pick presumptive Salmonella colonies, biochemical and serological confirmation. Modifications made at three stages of pick presumptive Salmonella colonies, biochemical confirmation and serological confirmation into a single stage of molecular testing to detect genes rfbE and, using tyv and prt primer. In summary, from 120 samples, the modified technique successfully identified 39 (32.5%) Salmonella enterica serovars Typhi and one Salmonella enterica serovars Paratyphi A (0, 83%) compare with 13 (10.8%) and negative result with conventional protocol. The modified were faster and more sensitive than conventional techniques.