Rapid identification of Salmonella enterica serovars Typhi and Salmonella enterica serovars Paratyphi A from chicken meat
D. Raharjo, R. Yulistiani, W. Setyarini
Oct 8, 2019
Citations
5
Citations
Journal
IOP Conference Series: Materials Science and Engineering
Full text
Semantic Scholar
Key Takeaway Key Takeaway: The modified culture-based method for Salmonella sp. identification in chicken meat samples is faster and more sensitive than conventional methods, improving food safety.
Abstract
Rapid diagnosis of pathogenic bacteria in food is closely related to safety. We modified a Culture-based methods for Salmonella sp. which rapidly identified Salmonella enterica serovars Typhi and Salmonella enterica serovars Paratyphi A from chicken meat samples. The World Health Organization method consists of six steps of the test, sequentially pre-enrichment, selective enrichment, selective diagnostic isolation, pick presumptive Salmonella colonies, biochemical and serological confirmation. Modifications made at three stages of pick presumptive Salmonella colonies, biochemical confirmation and serological confirmation into a single stage of molecular testing to detect genes rfbE and, using tyv and prt primer. In summary, from 120 samples, the modified technique successfully identified 39 (32.5%) Salmonella enterica serovars Typhi and one Salmonella enterica serovars Paratyphi A (0, 83%) compare with 13 (10.8%) and negative result with conventional protocol. The modified were faster and more sensitive than conventional techniques.