Nippon Jibiinkoka Gakkai Kaiho
Electric decalcification was applied to prepare the specimens of guinea pig labyrinth and influences of this procedure upon ganglionic cells of the acoustic nerve were histologically studied. The results were as follows: 1. It took more than 12 hours to secure perfect decalcification on animals weighted about 300 grams. 2. In the cases decalcificated for more than 48 hours the specimens were less stainable to thionin. 3. In the cases decalcificated for less than 36 hours no remarkable changes were observed with the exception that protoplasma and nuclei were more or less deeply stained, and Nissl's granules were well preserved, while in the cases of decalcification for 6 days, contraction of cells, interspace bilding around the protoplasma, pycnosis of the nuclei, disappearance of Nissl's gra-nules etc, were observed. It may be given as a conclusion that it is adequate to decalcify electrically from 12 to 36 hours when the labyrinth of guinea pig is examined.