Intracellular Leishmania amazonensis killing induced by the guanine nucleoside 8-bromoguanosine.

doi:10.1590/S0036-46651998000400006

Paper

Intracellular Leishmania amazonensis killing induced by the guanine nucleoside 8-bromoguanosine.

Published Jul 1, 1998 · S. Giorgio, S. Barão

Revista do Instituto de Medicina Tropical de Sao Paulo

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6

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Abstract

In this study we investigated the effect of 8-Bromoguanosine, an immunostimulatory compound, on the cytotoxicity of macrophages against Leishmania amazonensis in an in vitro system. The results showed that macrophages treated with 8-Bromoguanosine before or after infection are capable to reduce parasite load, as monitored by the number of amastigotes per macrophage and the percentage of infected cells (i.e. phagocytic index). Since 8-Bromoguanosine was not directly toxic to the promastigotes, it was concluded that the ribonucleoside induced macrophage activation. Presumably, 8-Bromoguanosine primed macrophages by inducing interferon alpha and beta which ultimately led to L. amazonensis amastigote killing. The results suggest that guanine ribonucleosides may be useful to treat infections with intracellular pathogens.

In Vitro Study

Study Snapshot

8-Bromoguanosine activates macrophages, leading to reduced Leishmania amazonensis parasite load by inducing interferon alpha and beta, suggesting potential for treating intracellular pathogen infections.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Paper

Intracellular Leishmania amazonensis killing induced by the guanine nucleoside 8-bromoguanosine.

References

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Citations

Bioactivity guided fractionation of Moringa oleifera Lam. flower targeting Leishmania donovani.

Moringa oleifera Lam. flower extract, particularly the ethyl acetate fraction, shows potential as a potent antileishmanial agent, reducing parasite burden in infected macrophages and in vivo mouse models of leishmaniasis.

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EFFECT OF HYPOXIA ON MACROPHAGE INFECTION BY LEISHMANIA AMAZONENSIS

Hypoxia can reduce macrophage susceptibility to parasitic infections, such as Leishmania amazonensis, without affecting phagocytic activity.

Efficacy of 8-Bromoguanosine against Murine Cutaneous Leishmaniasis Induced with Leishmania amazonensis

8-bromoguanosine (8Brguo) shows potential as a new leishmanial drug and immune modulator, with no apparent toxicity in a murine model of experimental cutaneous leishmaniasis.

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The adaptive MCMC algorithm based on the Metropolis-Hastings kernel and local curvature of a target density effectively samples densities with highly variable local curvatures, such as mixture distributions.