L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide--a chromogenic substrate for thiol proteinase assay.

doi:10.1016/0003-2697(84)90665-1

Paper

DOI: 10.1016/0003-2697(84)90665-1

L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide--a chromogenic substrate for thiol proteinase assay.

Published Dec 1, 1984 · Filippova IYu, E. N. Lysogorskaya, E. S. Oksenoit

Analytical biochemistry

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Abstract

Abstract hidden due to publisher request; this does not indicate any issues with the research. Click the full text link above to read the abstract and view the original source.

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Study Snapshot

PFLNA is a convenient chromogenic substrate for assaying thiol proteinases papain, ficin, and bromelain, providing 2- to 4-fold purification from commercial preparations.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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References

DOI: 10.1021/BI00662A014

Binding of chloromethyl ketone substrate analogues to crystalline papain.

The binding mode of papain substrate analogues in the active site groove is similar to that of proteases, suggesting a similar catalytic mechanism.

1977·291citations·J. Drenth et al.·Biochemistry

Biochemistry

DOI: 10.1042/BJ1240107

Kinetic specificity in papain-catalysed hydrolyses.

Papain's specificity for substrate peptide bonds and acyl residues is linked to a relaxation of protein conformation, facilitating binding and facilitating distortion.

1971·72citations·G. Lowe et al.·The Biochemical journal

The Biochemical journal

DOI: 10.1098/RSTB.1970.0024

Mapping the active site of papain with the aid of peptide substrates and inhibitors.

The active site of papain can be mapped using peptide substrates and inhibitors, providing insights into its size and specificity.

1970·352citations·A. Berger et al.·Philosophical transactions of the Royal Society of London. Series B, Biological sciences

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

DOI: 10.1016/0040-4020(76)80040-3

The Cysteine proteinases

Cysteine proteinases, found in mammals, plants, and bacteria, play diverse functions and have been studied extensively, with papain being the most studied and its tertiary structure known.

1976·160citations·G. Lowe·Tetrahedron

Tetrahedron

Citations

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2023·5citations·Huda Al-Badri et al.·Journal of dentistry

Journal of dentistry

DOI: 10.29011/2574-710x.010116

Glyco-polypeptides in Treating of Various Types of Late-Stage Refractory Solid Carcinoma in Humans-A Double-Blind Study-Case Report of 126 Patients: Complete Study Report of Phase II Clinical Trial

Glyco-polypeptides (Comosain) show promising anti-metastatic effects in various types of late-stage refractory solid cancer, with high-dose treatment yielding 52% complete and 27% partial responses.

2021·0citations·Benedict S Liao et al.·Journal of Oncology Research and Therapy

Journal of Oncology Research and Therapy

DOI: 10.29011/2574-710x.010115

Glyco-Polypeptides (Comosain) and Chimeric White Blood Cell Therapy in Late-Stage Refractory Solid Carcinoma of Lung, Prostatic and Bladder Cancers Review of 35 Cases

Glyco-polypeptides and chimeric white blood cell therapy show excellent anti-tumor effects in late-stage lung, prostate, and bladder cancers.

2021·0citations·Benedict S. Liao·Journal of Oncology Research and Therapy

Journal of Oncology Research and Therapy

DOI: 10.3390/app11188428

Recent Advances and Insights into Bromelain Processing, Pharmacokinetics and Therapeutic Uses

Bromelain has potential health benefits, but its composition varies across different plant parts and extraction methods, making it difficult to make evidence-based prescriptions.

2021·23citations·A. Colletti et al.·Applied Sciences

Applied Sciences

DOI: 10.1080/01496395.2021.1887235

Egg white protein polymer: an affinity matrix for protease enrichment and isolation

Egg white protein polymer effectively binds proteases, making it a cost-effective material for protease enrichment, isolation, and removal.

2021·1citation·Esra Menfaatli et al.·Separation Science and Technology

Separation Science and Technology