M. Ljaljevic, J. Ljaljević, C. Parker
May 1, 1968
Journal of immunology
Summary Conditions which led to inactivation of rabbit γG-globulin in direct passive cutaneous anaphylaxis (PCA) experiments in guinea pig skin also produced inactivation in reverse PCA experiments. Thus acetylation, carbamylation, picrylation and phthylation of reverse PCA antigens (rabbit Fc or dinitrophenylated rabbit γ-globulin) caused loss of skin-sensitizing activity whereas amidination did not. These results strongly favor the possibility that inactivation of skin-sensitizing activity is due to loss of fixation in guinea pig skin. In further support of this view no correlation was seen between complement-fixing activity and skin-sensitizing activity. Amidinated preparations of rabbit antibody which retained their ability to sensitize guinea pig skin fixed very little complement.