Modulation of cell-surface transferrin receptor by the imino sugar N-butyldeoxynojirimycin.

doi:10.1111/J.1432-1033.1992.TB17173.X

Paper

Modulation of cell-surface transferrin receptor by the imino sugar N-butyldeoxynojirimycin.

Published Aug 1, 1992 · F. Platt, G. Karlsson, G. Jacob

European journal of biochemistry

Q1 SJR score

20

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0

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Abstract

The imino sugar, N-butyldeoxynojirimycin, is an inhibitor of the glycoprotein-processing enzyme glucosidase I and exhibits anti-(human immunodeficiency virus) activity in vitro. We have investigated the effect(s) of this compound on cell-surface glycoproteins by flow cytometry. We observed selective modulation of the transferrin receptor in response to treatment with 0.5 mM N-butyldeoxynojirimycin resulting in reduced cell-surface transferrin-receptor expression. The receptor modulation was dose dependent, resulted in reduced 59Fe uptake by treated cells and was fully reversible within 24 h of culture in the absence of the compound. Pulse/chase analysis in conjunction with endoglycosidase-H digestion demonstrated that transferrin-receptor glycosylation was altered following N-butyldeoxynojirimycin treatment, which is compatible with glucosidase inhibition. In addition, modulation of transferrin receptor in response to N-butyldeoxynojirimycin was not confined to a single cell line, but was also observed with certain human lymphoid and myeloid cell lines. Mechanism(s) of action of the imino sugar resulting in reduced cell-surface transferrin-receptor expression are discussed.

In Vitro Study

Study Snapshot

N-butyldeoxynojirimycin selectively modulates the transferrin receptor, reducing its expression on cell-surface, potentially impacting HIV infection.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Paper

Modulation of cell-surface transferrin receptor by the imino sugar N-butyldeoxynojirimycin.

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Citations

AgOTf-catalyzed three-component coupling for the synthesis of C-alkynyl iminosugars

AgOTf-catalyzed three-component coupling effectively synthesizes C-alkynyl iminosugars, a key intermediate in biologically important natural products, under mild conditions.

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This one-pot method efficiently synthesizes diverse novel iminosugar C-alkynylglycosides, potentially useful for DNA cross-linking agents.

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Miglustat is a safe and well-tolerated drug for juvenile GM2 gangliosidosis, but its limited efficacy may lead to enzyme-enhancement therapy being a promising alternative treatment.

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