Neuropeptide Y inhibits cholinergic transmission in the isolated guinea pig colon: mediation through alpha-adrenergic receptors.

doi:10.1073/PNAS.84.7.2047

Paper

Neuropeptide Y inhibits cholinergic transmission in the isolated guinea pig colon: mediation through alpha-adrenergic receptors.

Published Apr 1, 1987 · J. Wiley, C. Owyang

Proceedings of the National Academy of Sciences of the United States of America

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56

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Abstract

Neuropeptide Y, a member of the pancreatic polypeptide family, caused dose-dependent relaxation of guinea pig colon longitudinal muscle. This inhibitory effect was unaffected by hexamethonium but was abolished by atropine and tetrodotoxin, suggesting that neuropeptide Y is acting via postganglionic cholinergic neurons. Studies utilizing alpha-adrenergic and dopamine receptor antagonists revealed that neuropeptide Y-mediated relaxation was blocked only by the alpha 2 antagonist yohimbine. Neuropeptide Y also antagonized muscle response to electric field stimulation that was reversed by yohimbine. Additional studies with muscle slices incubated with [3H]norepinephrine or [3H]choline showed that neuropeptide Y stimulated norepinephrine release from sympathetic nerves, which, in turn, inhibited the release of acetylcholine via alpha 2 receptors located on postganglionic nerves. This pathway provides a mechanism for neuropeptide modulation of classical neurotransmitter function.

In Vitro Study

Study Snapshot

Neuropeptide Y relaxes guinea pig colon muscle by inhibiting cholinergic transmission through alpha-adrenergic receptors, providing a mechanism for neuropeptide modulation of classical neurotransmitter function.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Paper

Neuropeptide Y inhibits cholinergic transmission in the isolated guinea pig colon: mediation through alpha-adrenergic receptors.

References

Citations

Mapping and quantifying neuropeptides in the enteric nervous system

Analytical challenges in studying neuropeptides in the enteric nervous system require further technical development to better understand their role in biological processes and potential therapeutic value.

Individual sympathetic postganglionic neurons coinnervate myenteric ganglia and smooth muscle layers in the gastrointestinal tract of the rat

Most sympathetic postganglionic neurons in the gastrointestinal tract of rats coinnervate both myenteric ganglia and smooth muscle, suggesting they control gastrointestinal muscle directly.

Neuropeptide Y: identification of the binding site.

The C-terminal tetrapeptide NPY 33-36 is essential for receptor recognition in neuropeptide Y, suggesting its role in neurotransmitter signaling.

Abnormality of Peptide YY and Pancreatic Polypeptide Immunoreactive Cells in Colonic Mucosa of Patients with Colonic Inertia

Patients with colonic inertia show abnormalities in peptide YY and pancreatic polypeptide immunoreactive cells, but not in enterochromaffin cells, suggesting a possible underlying cause.

Neuropeptide Y and Peptide YY Inhibit Excitatory Synaptic Transmission in the Rat Dorsal Motor Nucleus of the Vagus

Neuropeptide Y and peptide YY inhibit excitatory synaptic transmission in the rat dorsal motor nucleus of the vagus, potentially indicating functional differences in their roles within the dorsal vagal complex.