N-methyl-N'-nitro-N-nitrosoguanidine: reactions of possible significance to biological activity with mammalian cells.

Paper

N-methyl-N'-nitro-N-nitrosoguanidine: reactions of possible significance to biological activity with mammalian cells.

Published Jun 1, 1970 · T. Anderson, R. Burdon

Cancer research

Q1 SJR score

19

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0

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Semantic Scholar

Abstract

Mammalian cells exposed to N -methyl- N′ -nitro- N -nitrosoguanidine show an immediate inhibition of DNA, RNA, and protein synthesis, as judged by radioactive precursor incorporation. DNA synthesis as determined by thymidine-3H uptake is most sensitive in this respect. The inhibition is not due to impaired uptake or phosphorylation of precursor. Studies at the enzyme level show DNA polymerase to be inhibited by N -methyl- N′ -nitro- N -nitrosoguanidine in a dose-dependent manner. Experiments with exogenous thiols indicate that thiol groups of DNA polymerase may be targets for reaction with the guanidino radical resulting from N -methyl- N′ -nitro- N -nitrosoguanidine decomposition. Further studies suggest that these and other groups possibly involved in substrate binding may be altered and that enzyme kinetics favor a mixed mode of enzyme inhibition.

In Vitro Study

Study Snapshot

N-methyl-N′-nitro-N-nitrosoguanidine inhibits DNA, RNA, and protein synthesis in mammalian cells, potentially targeting DNA polymerase thiol groups for inhibition.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

N-methyl-N'-nitro-N-nitrosoguanidine: reactions of possible significance to biological activity with mammalian cells.

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