Rudolf H. Kaden M. D.
Mycopathologia et mycologia applicata
1) A simple technique for the preparation of dissolved autoclaved antigens and of sonic vibrated antigens, the immunisation of rabbits, and testing methods by precipitation in agar medium and in capillary tubes are described. 2) Five different strains ofSporotrichum belonging to three species,S. Schenckii, S. Beurmanni, andS. asteroides are studied as to their precipitin lines formation in agar medium. 3) The similarity in precipitin lines formation is interpreted to demonstrate the antigenic identity of these differentSporotrichum species. 4) Comparable results with minor differentiation but in major sensitivity are obtained by precipitin tests in capillary tubes. 5) Temperatures of 4° C are shown to stimulate intensity of precipitin lines. 6) Dissolved sonic vibrated antigens alter the precipitin formation by changing and intensifying the precipitin lines. 7) Absorption experiments with dissolved antigens give unsatisfactory results if precipitin tests in capillary tubes are used.