Journal of Shanghai Medica
Purpose To investigate the effect of p16 INK4A on the phenotype of A549 cell line. Methods A549 cell line was transfected with p16 INK4A and the stably transfected strain was identified with Northern blot and Southern blot.Cell cycle was analysied by flow cytometry.The phosphorylation state of retinoblastoma protein(Rb) and cell phenotype were determined by Western blot and SA-β-gal staining. Results Stable transfection of p16 INK4A was confirmed.Overexpression of p16 INK4A in A549 cell line caused a G1 phase arrest with the inhibition of phosphorylation of Rb protein.Significantly,the arrested cells displayed several phenotypic features that were considered as the characteristics of senescent cells.They had a large,flat appearance,and positive acidic β-galactosidase acivity. Conclusions Our results suggested that the re-expression of p16 INK4A in A549 cells caused an immediate cellular senescence-like phenotype.