Suppression of Adipogenesis by 5-Hydroxy-3,6,7,8,3',4'-Hexamethoxyflavone from Orange Peel in 3T3-L1 Cells.

doi:10.1089/jmf.2016.0060

Paper

Suppression of Adipogenesis by 5-Hydroxy-3,6,7,8,3',4'-Hexamethoxyflavone from Orange Peel in 3T3-L1 Cells.

Published Sep 1, 2016 · Yu Wang, Pei‐Sheng Lee, Yi-Fen Chen

Journal of medicinal food

Q3 SJR score

18

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0

Influential Citations

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Abstract

We reported previously that hydroxylated polymethoxyflavones (HPMFs) effectively suppressed obesity in high-fat-induced mouse. In this study, we further investigated the molecular mechanism of action of 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-OH-HxMF), one of major HPMFs in orange peel. Treatment of 5-OH-HxMF effectively inhibited lipid accumulation by 55-60% in a dose-dependent manner. The 5-OH-HxMF attenuated adipogenesis through downregulating adipogenesis-related transcription factors such as peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding proteins (C/EBPs), as well as downstream target fatty acid synthase and acetyl-CoA carboxylase (ACC). 5-OH-HxMF activated adenosine monophosphate-activated protein kinase signaling and silent mating type information regulation 1 (SIRTUIN 1 or SIRT1) in 3T3-L1 adipocytes to decrease lipid accumulation. In addition, the inhibition rate of lipid accumulation was compared between 5-OH-HxMF and 3,5,6,7,8,3',4'-heptamethoxyflavone (HpMF). 5-OH-HxMF inhibited lipid accumulation 15-20% more than HpMF did, indicating that hydroxyl group at position 5 can be a key factor in the suppression of adipogenesis.

In Vitro Study

Study Snapshot

5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-OH-HxMF) from orange peel effectively inhibits lipid accumulation by 55-60% in 3T3-L1 cells, with the hydroxyl group at position 5 being a key factor in its

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Paper

Suppression of Adipogenesis by 5-Hydroxy-3,6,7,8,3',4'-Hexamethoxyflavone from Orange Peel in 3T3-L1 Cells.

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Citations

Antioxidative, Metabolic and Vascular Medicinal Potentials of Natural Products in the Non-Edible Wastes of Fruits Belonging to the Citrus and Prunus Genera: A Review

Non-edible wastes (peel and seed) from Citrus and Prunus fruits show potential medicinal benefits for improving oxidative, metabolic, and vascular health.

Hydroxylated polymethoxyflavones reduce the activity of pancreatic lipase, inhibit adipogenesis and enhance lipolysis in 3T3-L1 mouse embryonic fibroblast cells.

Hydroxylated polymethoxyflavones (HPMFs) have anti-obesity effects by reducing pancreatic lipase activity, inhibiting adipogenesis, and enhancing lipolysis in 3T3-L1 mouse embryonic fibroblast cells.

Anti-pancreatic lipase and anti-adipogenic effects of 5, 7, 3',4',5' -pentamethoxy and 6, 2',4'-trimethoxy flavone - An In vitro study.

Both 5,7,3′,4′,5′-pentamethoxyflavone (PMF) and 6,2′,4′-trimethoxyflavone (TMF) possess anti-obesity activities, with PMF being a more potent inhibitor of pancreatic

Beneficial Effects of Bioactive Compounds Obtained from Agro-Industrial By-Products on Obesity and Metabolic Syndrome Components

Bioactive compounds obtained from agro-industrial by-products show potential in preventing and treating obesity and metabolic syndrome components.

A Network Pharmacology-Based Study on Irritable Bowel Syndrome Prevention and Treatment Utilizing Shenling Baizhu Powder

Shenling Baizhu Powder effectively treats irritable bowel syndrome by influencing multiple targets and pathways, with the TNF signaling pathway being the most significant.