A. Pospelova, N. V. Gorelova
May 1, 1978
Bulletin of Experimental Biology and Medicine
Protein synthesis was studied by determining incorporation of [3H]glycine in cells of the regenerating rat liver. The rate of incorporation of [3H]glycine into total liver proteins, proteins of the microsomal fraction and of the hyaloplasm, and proteins of the plasma membranes, soluble and insoluble in 0.05 M K2CO3 was determined. The rate of incorporation of [3H]glycine into soluble proteins of the plasma membranes reached a maximum 1 h after partial hepatectomy. The maximal rate of synthesis of proteins of the other fractions occurred at the end of the g1 period. The sharp increase in the rate of synthesis of plasma membrane proteins soluble in 0.05 M K2CO3 is evidently one of the earliest biochemical events in cells of the regenerating liver preparing to divide.