Targeting Conserved Genes in Fusarium Species.
Published 2017 · J. Gil-Serna, B. Patiño, M. Jurado
Methods in molecular biology
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Abstract
Fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by F. verticillioides and F. proliferatum. Additionally, both are pathogens of maize and other cereals. We describe two highly sensitive, rapid, and species-specific PCR protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. The specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rDNA unit which highly improves the sensitivity of the PCR assay in comparison with single-copy target regions.
Study Snapshot
Key takeawayHighly sensitive, rapid, and species-specific PCR protocols enable detection and discrimination of Fusarium species in cereal flour or grain samples, improving food safety.
PopulationOlder adults (50-71 years)
Sample size24
MethodsObservational
OutcomesBody Mass Index projections
ResultsSocial networks mitigate obesity in older groups.