Deng Gang, S. Yang, Mu Jun-jie
Mar 20, 2006
In order to find the cause of different transactivate abilities among JDV, BIV and HIV-1 Tat, four chimeric Tat cDNA expression constructs, JH, HJ, JB and BJ, were generated with the crossover points at the boundary of activation and binding domains based on functional domain division of HIV-1 Tat and amino acid sequence comparision among HIV-1, JDV and BIV Tat. These chimeric Tat proteins were co-transfected with JDV, BIV and HIV-1 LTR report plasmids in Hela. Transient assay showed that different transactivate abilities among JDV, BIV and HIV-1 Tat were mainly caused by different binding abilities of their binding domains. We further excluded some possibilities that may cause the poor transactivate ability of JH on HIV-1 LTR, such as incomplete functional domains and the lack of related cytokines.