Twelve polymorphic expressed sequence tags-derived markers for Plasmopara halstedii, the causal agent of sunflower downy mildew
X. Giresse, D. T. D. Labrouhe, S. Richard-Cervera
Nov 1, 2007
Citations
31
Citations
Journal
Molecular Ecology Notes
Full text
Semantic Scholar
Key Takeaway Key Takeaway: Plasmopara halstedii, the causal agent of sunflower downy mildew, shows high genetic diversity and is likely a selfing species, with markers useful for detecting genetic variations among French and Russian populations.
Abstract
Twelve expressed sequence tags-derived markers were isolated from Plasmopara halstedii (Oomycetes), the causal agent of sunflower downy mildew. A total of 25 single nucleotide polymorphisms and five indels were detected by single-strand conformation polymorphism analysis and developed for high-throughput genotyping of 32 isolates. There was a high level of genetic diversity (HE = 0.484). Observed heterozygosity ranged from 0 to 0.143 indicating that P. halstedii is probably a selfing species. These markers were also useful in detecting significant genetic variations among French populations (FST = 0.193) and between French and Russian populations (FST = 0.23). Cross-amplification tests on three closely related species indicated that no loci amplified in other Oomycete species.