Ultraviolet-Visible (UV-VIS) Spectroscopy
Published 2019 · M. Akash, K. Rehman
11
Citations
0
Influential Citations
Abstract
UV-VIS spectroscopy is considered as the most important spectrophotometric technique that is most widely used for the analysis of variety of compounds. This technique works on the basis of the measurement of interaction of electromagnetic radiations (EMR) with matter at particular wavelength. In this chapter, we have briefly described the UV-VIS spectroscopy by covering the fundamentals of UV-VIS spectroscopy, origin of spectra along with the types of electronic transitions. We have also described the effect of solvents on the absorption spectra of analyte. For proper working of UV-VIS spectroscopy and to get accurate results, it is very important to understand the components of UV-VIS spectroscopy and their individual role in the proper functioning of UV-VIS spectrophotometer. In UV-VIS spectroscopy, absorption of light is the basic phenomenon and we have also described the various absorbance laws on which UV-VIS spectroscopy works. At the end of this chapter, we have also discussed the various terms that are used in this spectroscopy along with the diverse applications of this analytical technique.