Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery.
D. Chakraborty, Masanaga Muto, M. Soares
Dec 20, 2017
Citations
9
Citations
Journal
Bio-protocol
Full text
Semantic Scholar
Key Takeaway Key Takeaway: Lentiviral gene delivery effectively and specifically manipulates rat trophoblast cell lineage, potentially impacting embryo development and pregnancy outcomes.
Abstract
In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of 'gain-of-function' and 'loss-of-function' blastocyst manipulations can be evaluated with in vitro outgrowth assays or following transfer to pseudopregnant rats.
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