Biotin supplementation may lead to inaccurate results in various laboratory tests, potentially causing patient safety risks.

Biotin interference: Underrecognized patient safety risk in laboratory testing.

This paper reviews methods for assessing biotin levels in foods, blood, serum/plasma, and urine, and highlights the importance of detecting functional biotin deficiency using multiple carboxylase deficiency.

Methods for assessment of biotin (Vitamin B7)

Magnesium biotinate is a bioavailable form of biotin, with increasing blood levels associated with increasing dose levels in healthy female subjects.

Pharmacokinetics of a Novel Form of Biotin, Magnesium Biotinate, in Healthy Subjects (P06-027-19).

Biotin supplements, often marketed as beauty products, can interfere with diagnostic tests, potentially causing inaccurate results.

Biotin Interference in Diagnostic Tests.

Biotin interference in immunoassays can cause falsely low or high results, and laboratory scientists must manage this issue to prevent incorrect patient results.

Biotin interference in immunoassay: a review for the laboratory scientist

Biotin supplements can cause erroneous 25(OH)D and iPTH results in end-stage renal disease patients, potentially impacting patient care.

Biotin supplement interference with immunoassays for parathyroid hormone and 25-hydroxyvitamin D in a patient with metabolic bone disease on maintenance hemodialysis.

Pharmacological doses of biotin affect glucose and lipid metabolism, hypertension, reproduction, development, and immunity in animals, with potential toxic effects still poorly studied.

Pharmacological Effects of Biotin in Animals.

Biotin supplementation may reduce multiple sclerosis symptoms in some patients, but its effectiveness in healthy individuals with no deficiency remains unclear.

Biotin: From Supplement to Therapy

Biotin ingestion in moderate to high doses can cause immunoassay interference, potentially leading to falsely high or low values in various tests, requiring awareness for accurate diagnosis and treatment.

Factitious Graves' Disease Due to Biotin Immunoassay Interference-A Case and Review of the Literature.

Adding vitamin B7 in different concentrations to the diet of broiler chickens exposed to oxidative stress improves productivity and some physiological traits compared to negative and positive control treatments.

The Effectiveness of Biotin (Vitamin B7) Added to the Diet in Improving the Efficiency of Productivity, and Some Physiological Traits for Broiler Chickens (Ross-308) Exposed to Oxidative Stress.

This study presents a method for quantifying biotin in plasma and serum samples using column switching liquid chromatography-tandem mass spectrometry, and explores its effects on biotin-streptavidin based tests, to determine if high biotin intake may interfere with diagnostic assays

Quantification of biotin in plasma samples by column switching liquid chromatography – tandem mass spectrometry

Biotin supplementation's popularity is disproportionate to its insufficient clinical evidence supporting its efficacy in hair improvement, with no randomized controlled trials or clinical trials examining its effect on hair quantity or quality.

The Infatuation With Biotin Supplementation: Is There Truth Behind Its Rising Popularity? A Comparative Analysis of Clinical Efficacy versus Social Popularity.

High doses of biotin may cause erroneous immunoassay results, highlighting the importance of interpreting laboratory results in the context of patient's clinical state.

Biotin treatment causing erroneous immunoassay results: A word of caution for clinicians.

Biotin is essential for life and plays a crucial role in fatty acid synthesis, gluconeogenesis, and amino acid metabolism, with no known toxicity.

Biotin: Pharmacology, Pathophysiology, and Assessment of Biotin Status

Biotin-naphthoquinone, a chemiluminescence sensor, can effectively detect biotin in vitamin B7 tablets with good recovery and precision.

Biotinylated Quinone as a Chemiluminescence Sensor for Biotin-Avidin Interaction and Biotin Detection Application

Chronic alcohol exposure inhibits gut vitamin B7 uptake through epigenetic mechanisms and alcohol metabolites, affecting biotin absorption and SMVT expression.

Effect of chronic alcohol exposure on gut vitamin B7 uptake: Involvement of epigenetic mechanisms and effect of alcohol metabolites.

Biotin deficiency increases inflammation in CD4+ T cells, contributing to inflammation associated with biotin deficiency.

Biotin Deficiency Induces Th1- and Th17-Mediated Proinflammatory Responses in Human CD4+ T Lymphocytes via Activation of the mTOR Signaling Pathway

Biotin deficiency enhances the inflammatory responses of human dendritic cells, potentially mediating the inflammation associated with biotin deficiency.

Biotin deficiency enhances the inflammatory response of human dendritic cells.

The HPLC method is optimal for determining biotin in nutrients and samples, with statistical parameters comparing favorably to other methods.

Appraisal of Techniques, Investigation and Analysis of Vitamin (B7) Biotin

Biotin can be reduced to its carboxylate anion and dihydrogen by electrochemical means, and its methyl ester can be produced in excellent yield (91%).

The electrochemical reduction of biotin (vitamin B7) and conversion into its ester

Some studies suggest biotin is safe, while other studies highlight potential risks such as interference with laboratory tests and possible toxic effects at high doses.