2,3-butanedione as a photosensitizing agent: application to alpha-amino acids and alpha-chymotrypsin.

doi:10.1139/O79-168

Paper

2,3-butanedione as a photosensitizing agent: application to alpha-amino acids and alpha-chymotrypsin.

Published Nov 1, 1979 · H. Fliss, T. Viswanatha

Canadian journal of biochemistry

26

Citations

0

Influential Citations

Full text

Abstract

2,3-Butanedione sensitized the rapid photodestruction of free alpha-amino acids, and the photoinactivation of alpha-chymotrypsin, in the presence of ultraviolet light and oxygen. These reactions showed "pseudo-first-order" kinetics at 2,3-butanedione concentrations approximating those employed for the chemical modification of arginine residues in proteins. The photoreactions were inhibited in anoxic media or in the presence of azide; findings were consistent with a singlet oxygen mechanism for these reactions. No enhancement in the rate of reaction was observed in D2O. The rate of 2,3-butanedione-sensitized photodestruction of free amino acids increased with increasing pH. However, the rate constants for the photosensitized inactivation of alpha-chymotrypsin, as well as those for the photodestruction of the tryptophan residues of this enzyme, decreased linearly with increasing pH.

In Vitro Study

Study Snapshot

2,3-butanedione effectively photosensitizes alpha-amino acid destruction and alpha-chymotrypsin inactivation, with a singlet oxygen mechanism for these reactions.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

2,3-butanedione as a photosensitizing agent: application to alpha-amino acids and alpha-chymotrypsin.

References

Citations